Journal: iScience

Article Title: AMPylation profiling during neuronal differentiation reveals extensive variation on lysosomal proteins

doi: 10.1016/j.isci.2021.103521

Figure Lengend Snippet:

Article Snippet: Recombinant human LIF , Alomone Labs , Cat# L-200.

Techniques: Recombinant, Protease Inhibitor, Western Blot, Bicinchoninic Acid Protein Assay, Software

Journal: Communications biology

Article Title: Cytokimera GIL-11 rescued IL-6R deficient mice from partial hepatectomy-induced death by signaling via non-natural gp130:LIFR:IL-11R complexes.

doi: 10.1038/s42003-023-04768-4

Figure Lengend Snippet: Fig. 1 Design of the cytokimera GIL-11. a schematic illustration of the tetrameric and hexameric IL-11:IL-11R:gp130 receptor complex possibilities. IL-11 initially binds to IL-11R via site I. gp130 is recruited into this complex via major interactions between site III of IL-11 and D1 (Ig-like) of gp130 and site II of IL- 11 and D2/D3 (cytokine binding module (CBM)) of gp130. b schematic illustration of the trimeric LIF:gp130:LIFR complex. LIF binds via site III to D3/D4 of LIFR and via site II to D2/D3 of gp130. c schematic illustration of the tetrameric GIL-11:IL-11R:gp130:LIFR complex. GIL-11 binds via site III to D3/D4 of LIFR, via site II to D2/D3 of gp130 and via site I to D2/D3 of IL-11R. d schematic illustration (left) and space fill model (right) of the tetrameric GIL-11:IL- 11R:LIFR:gp130 (PDB 6O4P;11 2Q7N;83 1P9M10). GIL-11 binds via site I to IL-11R, via site II to gp130 and via site III to LIFR. e Combined transparent space fill/ band model of GIL-11, red: IL-11, green: exchanged region by LIF. f Structure-based schematic sequence alignment of hIL-11 (PDB 6O4O11), hLIF (PDB 1PVH33) and the resulting GIL-11. Site IIIa,b,c residues are highlighted in green and red boxes.

Article Snippet: Recombinant human OSM (catalog no. 295-OM) and recombinant human LIF (catalog no. 7734-LF) were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Binding Assay, Sequencing

Journal: Molecular Medicine Reports

Article Title: Expression and effects of leukemia inhibitory factor on nucleus pulposus degeneration

doi: 10.3892/mmr.2019.9874

Figure Lengend Snippet: Protein expression of aggrecan and COL2α1 in human nucleus pulposus cells stimulated with various concentrations of LIF at different durations. (A and B) Aggrecan and COL2α1 protein expression in human nucleus pulposus cells treated with various concentrations of recombinant human LIF protein. (C and D) Protein expression levels of aggrecan and COL2α1 in human nucleus pulposus cells treated with 100 ng/ml recombinant human LIF protein for various durations. (E) Toluidine blue staining following treatment of human nucleus pulposus cells with 0 or 100 ng/ml recombinant human LIF protein for 48 h. Data are presented as the means ± standard deviation. *P<0.05. COL2α1, collagen type II α1; LIF, leukemia inhibitory factor.

Article Snippet: Recombinant Human LIF Protein was purchased from Novus Biologicals, LLC (Littleton, CO, USA).

Techniques: Expressing, Recombinant, Staining, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Expression and effects of leukemia inhibitory factor on nucleus pulposus degeneration

doi: 10.3892/mmr.2019.9874

Figure Lengend Snippet: Effects of LIF on the proliferation and apoptosis of nucleus pulposus cells. (A and B) Apoptotic rate of human nucleus pulposus cells treated with various concentrations of recombinant human LIF. (C) Proliferation of human nucleus pulposus cells treated with various concentrations of recombinant human LIF. Data are presented as the means ± standard deviation. *P<0.05. FITC, fluorescein isothiocyanate; LIF, leukemia inhibitory factor; OD, optical density.

Article Snippet: Recombinant Human LIF Protein was purchased from Novus Biologicals, LLC (Littleton, CO, USA).

Techniques: Recombinant, Standard Deviation